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Section ¢ñ Theories and Strategies of Biochemistry and Molecular Biology Technology in Medical ResearchChapter 1 Medical Application of Biochemistry and Molecular Biology Technology 1.1 Basic Objectives of Biochemistry and Molecular Biology Technology 1.2 Application of Biochemistry and Molecular Biology Technology in Medical ResearchChapter 2 The Common Research Techniques of Gene 2.1 Nucleic Acid Hybridization and DNA Microarray 2.2 DNA Sequencing 2.3 Polymerase Chain Reaction (PCR) 2.4 Recombinant DNA Technology 2.5 The Research Strategies and Methods for Gene Transcription RegulationChapter 3 The Common Research Techniques of Protein 3.1 Quantification and Identification of Protein 3.2 Methods and Basic Principles of Protein Purification 3.3 Protein Chemical Modification and Spatial Structure Analysis 3.4 Protein Interaction Research Methods 3.5 Proteomies Research 3.6 Research Technology of EnzymologySection ¢ò Basic Biochemical TechniquesChapter 4 Basic Operation of Biochemical Experiments 4.1 Solution Preparation 4.2 Pipettes Operation 4.3 Centrifuge OperationChapter 5 Spectrophotometry 5.1 Basic Concepts and Principle of Spectrophotometry 5.2 Measurement of Light Absorption 5.3 Applications of Spectrophotometry Experiment 1 Quantitative Analysis of Protein Experiment 2 Determination of Blood Glucose Level Regulated by HormonesChapter 6 Electrophoresis 6.1 Basic Principles of Electrophoresis 6.2 Impact Factors of Electrophoresis 6.3 Detection of Components after Electrophoretic Separation 6.4 Special Electrophoretic Techniques and Applications Experiment 3 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis Experiment 4 Separation of Serum Lipoproteins by Agarose Gel Electrophoresis Experiment 5 Proteins Separation by SDS-Polyacrylamide Gel ElectrophoresisChapter 7 Chromatography 7.1 Fundamental Principles of Chromatography 7.2 Commonly Used Chromatographic Techniques Experiment 6 Separation of Mixed Amino Acids by Cation Exchange Chromatography Experiment 7 Separation of Hemoglobin and Dinitrophenyl (DNP) -glutamate by Gel Filtration ChromatographySection ¢ó Integrative ExperimentsChapter 8 Enzyme Analysis 8.1 The Activity of Enzyme 8.2 Enzyme Kinetics ( Factors Affecting Reaction Velocity) 8.3 Enzymatic Analysis Experiment 8 Assay the Activity of Alanine Aminotransferase (ALT) in Serum ( Mohun's Method) Experiment 9 Effect of Substrate Concentration on Enzyme Activity-Determining Km Value for Alkaline Phosphatase Experiment 10 Enzymatic Endpoint Method-Determination of Total Cholesterol, Triglycerides and Glucose Levels in SerumChapter 9 Isolation, Purification and Identification of Protein 9.1 Procedure of Protein Isolation 9.2 The Selective Purification Steps 9.3 Crystallization, Concentration, Drying and Storage of Protein 9.4 Identification and Analysis of Protein Experiment 11 Isolation and Identification of Serum IgG Experiment 12 Purification and Identification of Glutathione S-Transferase Fusion ProteinChapter 10 Isolation, Purification and Identification of Nucleic Acids 10.1 Preparation Principle and Procedure of Nucleic Acids 10.2 Identification and Analysis of Nucleic Acids Experiment 13 Isolation and Identification of Eukaryotic Genomie DNA Experiment 14 SDS-Alkaline Lysis Preparation of Plasmid DNA Experiment 15 Isolation and Identification of Total RNA in Tissues/Cells Experiment 16 Polymerase Chain Reaction (PCR) and Reverse Transeription-PCRChapter 11 Genetic Engineering 11.1 The Process of Genetic Engineering 11.2 Expression of Foreign Genes and Purification of Recombinant Proteins Experiment 17 DNA Cloning Experiment 18 Expression of Exogenous Gene in E.eoli and Purification of His-tag ProteinsChapter 12 Innovation Experiment Design and Application Experiment 19 Separation and Identification of Alkaline Phosphatase Experiment 20 Lactate Dehydrogenase (LDH) Analysis Experiment 21 Protein Markers Exploration by Two-dimensional EleetrophoresisReference