µ°°×ÖÊ×éѧÑо¿·½·¨ÓëʵÑé·½°¸

Ŀ¼ Ç°ÑÔ v ׫¸åÈË ix **²¿·Ö ÒýÑÔ 1. µ°°×ÖÊ×éѧ¼ò½é 3 Friedrich Lottspeich µÚ¶þ²¿·Ö µçÓ¾·ÖÀë 2. ¸ß·Ö±æÂʶþάµçÓ¾ 13 Walter Weiss and Angelika Gorg 3. ·Ç¾­µä¶þάµçÓ¾ 33 JacquelineBurre£¬Ilka Wittig£¬and Hermann Schagger 4. µ°°×Öʼì²âÓ붨Á¿¼¼ÊõÓÃÓÚ»ùÓÚÄý½ºµÄµ°°×ÖÊ×éѧ·ÖÎö 59 Walter Weiss£¬Florian Weiland£¬and Angelika Gorg µÚÈý²¿·Ö ÖÊÆ׺ʹ®ÁªÖÊÆ×µÄÓ¦Óà 5. »ùÖʸ¨Öú¼¤¹â½âÎüµçÀëÖÊÆ× 85 Rainer Cramer 6. ëϸ¹ÜµçÓ¾ÖÊÆ×ÁªÓÃÓ¦ÓÃÓÚÈËÄòÒºµ°°×ÖÊ×éѧ·ÖÎöÓëÉúÎï±êÖ¾Îï·¢ÏÖ 105 PetraZurbig£¬Eric Schiffer£¬and Harald Mischak 7. ¶àëÄÄÉÉý¼¶ÒºÏàÉ«Æ×Ö¸ÄÏ 123 Thomas Frohlich and Georg J.Arnold 8. ¶àάµ°°×¼ø¶¨¼¼Êõ 143 Katharina Lohrig and Dirk Wolters 9. ëĶÎΪÖÐÐĵĵ°°×ÖÊ×éѧ¼¼Êõ·ÖÎöѪС°åµ°°× 155 Oliver Simon£¬Stefanie Wortelkamp£¬and Albert Sickmann 10. ¸ß·Ö±æÂÊÖÊÆ×µ°°×ÖÊ×é·ÖÎö¼¼Êõ¼ø¶¨Ð¡ÊóС³¦20Sµ°°×ÖÊ×é·Ö×Ó×é³É 173 Reinhold Weber£¬Regina Preywisch£¬Nikolay Youhnovski£¬Marcus Groettrup£¬and Michael Przybylski µÚËIJ¿·Ö µ°°×ÖÊ×éѧ¶¨Á¿¼¼Êõ 11. »ùÓÚÒºÏàÉ«Æ×ÖÊÆ×ÁªÓõĶ¨Á¿µ°°×ÖÊ×éѧ 189 Michael W.Linscheid£¬Robert Ahrends£¬Stefan Pieper£¬and Andreas Kuhn 12. ½ºÄÚøÇÐͬλËرê¼Çµ°°×Öʼ¼ÊõÓÃÓÚÏà¶Ô¶¨Á¿ 207 Carla Schmidt and Henning Urlaub 13.µçÅçÎíÖÊÆ×¼¼ÊõÓÃÓÚѪ½¬µ°°×ÖÊ×鶨Á¿·ÖÎö 227 Hong Wang and Sam Hanash µÚÎ岿·Ö ÖÊÆ×Êý¾Ý½â¶Á 14. Ëã·¨ºÍÊý¾Ý¿â 245 Lennart Martens and Rolf Apweiler 15. Äñǹ·¨µ°°×Öʼø¶¨ºÍÖÊÆ×·¨µ°°×Öʶ¨Á¿ 261 Bingwen Lu£¬Tao Xu£¬Sung Kyu Park£¬and John R.Yates III µÚÁù²¿·Ö µ°°×ÖÊÐÞÊηÖÎö 16. ÄÔµ°°×Ñõ»¯ÐÞÊε°°×ÖÊ×éѧ¼ø¶¨ 291 Rukhsana Sultana£¬Marz ia Perluigi£¬and D.Allan Butter field 17.ͬλËرê¼Ç¡¢Á×Ëữ¶àëÄÇ׺͸»¼¯¼¼ÊõÓÃÓÚÒºÏàÉ«Æ×-´®ÁªÖÊÆ×µ°°×ÖÊ×é·ÖÎö 303 Uma Kota£¬Ko-yi Chien£¬and Michael B.Goshe µÚÆß²¿·Ö ÑÇϸ°ûµ°°×ÖÊ×éѧ 18. ϸ°ûÆ÷µ°°×ÖÊ×éѧ£ºÌìÈ»µ°°×½ºÄÚøÇÐɸѡ¼õÉÙÑù±¾µÄ¸´ÔÓÐÔ 325 Veronika Reisinger and Lutz A.Eichacker 19. ¾ÛºÏÎïË«Ë®ÏàϵͳÄæÁ÷·ÖÅä·¨·ÖÀëÉñ¾­ÏµÍ³ÖÊĤ 335 Jens Schindler and Hans Gerd NothWang 20. ÒºÏàÉ«Æ×´®ÁªÖÊÆ×¼¼Êõ½øÐÐÄâÄϽ浰°×ÖÊ×é·ÖÎöµÄÖÊĤµ°°×¸»¼¯ºÍÖƱ¸ 341 Srijeet K.Mitra£¬Steven D.Clouse£¬and Michael B.Goshe µÚ°Ë²¿·Ö µ°°×ÖÊÏ໥×÷Ó÷ÖÎö 21.Strep/FLAG(SF)-´®ÁªÇ׺ʹ¿»¯±êÇ©ÓÃÓÚ²¸È鶯Îïϸ°ûµ°°×Öʸ´ºÏÎï´®ÁªÇ׺ʹ¿»¯ 359 Christian Johannes Gloeckner£¬Karsten Boldt£¬Annette Schumacher£¬and Marius Ueffing 22. ˳Ðò¶àëÄÇ׺ʹ¿»¯ÏµÍ³·ÖÀëºÍ¼ø¶¨´ó³¦¸Ë¾úµ°°×Öʸ´ºÏÎï 373 Mohan Babu£¬Gareth Butland£¬Oxana Pogoutse£¬Joyce Li£¬Jack F.Greenblatt£¬and AndreWEmili 23. ÉúÎïÐÅϢѧ·½·¨·ÖÎöµ°°×ÖÊÏ໥×÷Óà 401 Beate Kruger and Thomas Dandekar Ë÷Òý 433 (Ëο­ ÕÅ´ºÐã Òë) Contents Preface. v Contributors. ix PART I INTRODUCTION 1. Introduction to Proteomics 3 Friedrich Lottspeich PART II ELECTROPHORETIC SEPARATIONS 2. High-Resolution Two-Dimensional Electrophoresis 13 Walter Weiss and Angelika Gorg 3. Non-classical 2-D Electrophoresis 33 Jacqueline Burre£¬Ilka Wittig£¬and Hermann Sch£¿gger 4. Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis 59 Walter Weiss£¬Florian Weiland£¬and Angelika Gorg PART III MASS SPECTROMETRY AND TANDEM MASS SPECTROMETRY APPLICATIONS 5. MALDI MS 85 Rainer Cramer 6. Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery 105 Petra Zurbig£¬Eric Schiffer£¬and Harald Mischak 7. A Newcomer's Guide to Nano-Liquid-Chromatography of Peptides 123 Thomas Frohlich and Georg J. Arnold 8. Multidimensional Protein Identification Technology 143 Katharina Lohrig and Dirk Wolters 9. Characterization of Platelet Proteins Using Peptide Centric Proteomics 155 Oliver Simon£¬Stefanie Wortelkamp£¬and Albert Sickmann 10. Identification of the Molecular Composition of the 20S Proteasome of Mouse Intestine by High-Resolution Mass Spectrometric Proteome Analysis 173 Reinhold Weber£¬Regina Preywisch£¬Nikolay Youhnovski£¬Marcus Groettrup£¬and Michael Przybylski PART IV QUANTITATIVE PROTEOMICS 11. Liquid Chromatography-Mass Spectrometry-Based Quantitative Proteomics. 189 Michael W. Linscheid£¬Robert Ahrends£¬Stefan Pieper£¬and Andreas Kuhn 12. iTRAQ-Labeling of In-Gel Digested Proteins for Relative Quantification 207 Carla Schmidt and Henning Urlaub 13. Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis 227 Hong Wang and Sam Hanash PART V INTERPRETATION OF MASS SPECTROMETRY DATA 14. Algorithms and Databases 245 Lennart Martens and Rolf Apweiler 15. Shotgun Protein Identification and Quantification by Mass Spectrometry 261 Bingwen Lu£¬Tao Xu£¬Sung Kyu Park£¬and John R. Yates III PART VI ANALYSIS OF PROTEIN MODIFICATIONS 16. Proteomics Identification of Oxidatively Modified Proteins in Brain 291 Rukhsana Sultana£¬Marzia Perluigi£¬and D. Allan Butterfield 17. Isotope-Labeling and Affinity Enrichment of Phosphopeptides for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry 303 Uma Kota£¬Ko-yi Chien£¬and Michael B. Goshe PART VII SUBCELLULAR PROTEOMICS 18. Organelle Proteomics£ºReduction of Sample Complexity by Enzymatic In-Gel Selection of Native Proteins 325 Veronika Reisinger and Lutz A. Eichacker 19. Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems 335 Jens Schindler and Hans Gerd Nothwang 20. Enrichment and Preparation of Plasma Membrane Proteins from Arabidopsis thaliana for Global Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry 341 Srijeet K. Mitra£¬Steven D